Try following sequence, which may give you a relatively quick purification.

Q-sepharose (pH 7-7.5)--->hydrophobic interaction (phenyl-sepharose or butyl-sepharose starting at 0.5-1M ammonium sulfate)--->Sephadex 200 (longer than 2 meters)--->final clean up with another Q-sepharose (sharrow salt gradient, better with a FPLC).

You may also try dye affinity columns with a kit from Sigma to find one with certain degree of binding. How to incorporate different columns is the key and desalt and change of buffer is very important as well.

Of course, every protein is different and protein purification is very time-consuming. Anyway, good luck!