According to the theory of relativity, due to their constant movement and height relative to the Earth-centered inertial reference frame, the clocks on the satellites are affected by their speed (special relativity) as well as their gravitational potential (general relativity). For the GPS satellites, general relativity predicts that the atomic clocks at GPS orbital altitudes will tick more rapidly, by about 45.9 microseconds (μs) per day, because they are in a weaker gravitational field than atomic clocks on Earth's surface. Special relativity predicts that atomic clocks moving at GPS orbital speeds will tick more slowly than stationary ground clocks by about 7.2 μs per day. When combined, the discrepancy is about 38 microseconds per day; a difference of 4.465 parts in 1010.[18]. To account for this, the frequency standard onboard each satellite is given a rate offset prior to launch, making it run slightly slower than the desired frequency on Earth; specifically, at 10.22999999543 MHz instead of 10.23 MHz.[19]

The atomic clocks on board the GPS satellites are precisely tuned, making the system a practical engineering application of the scientific theory of relativity in a real-world environment.