转基因大豆喂食小鼠使其肝细胞核结构改变?



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送交者: dreamer 于 2005-3-05, 13:50:59:

看到一则旧闻,
http://www.bioon.com/biology/advance/Class18/200406/48200.html
意大利乌尔比诺大学的科学家们2004年6月初通过试验表明,转基因大豆可以改变小白鼠的肝脏结构。
在给小白鼠喂食转基因大豆后,意科学家们对小白鼠的肝脏细胞进行了单细胞结构和免疫细胞化学分析。分析后发现,用转基因大豆喂养的小白鼠的肝细胞核出现了不规则的变化,本来圆润的肝细胞核经过一段时间后变成了带有无数纤维质的不规则形状。这个变化说明了小白鼠体内的新陈代谢加快。
科学家们由此得出结论,经过喂食转基因大豆,小白鼠体内的肝细胞结构可以发生变化。而这种变化的深层机理还有待于科学家们进一步的研究。因为还缺乏足够的科学证据,目前科学家们还不能得出转基因食品可以直接危害健康的结论。

检索到了原文,还没看,先贴了。
Ultrastructural morphometrical and immunocytochemical analyses of hepatocyte nuclei from mice fed on genetically modified soybean
Author(s): Malatesta M, Caporaloni C, Gavaudan S, Rocchi MBL, Serafini S, Tiberi C, Gazzanelli G
Source: CELL STRUCTURE AND FUNCTION 27 (4): 173-180 AUG 2002
Document Type: Article
Language: English
Cited References: 29 Times Cited: 3
Abstract: No direct evidence that genetically modified (GM) food may represent a possible danger for health has been reported so far; however, the scientific literature in this field is still quite poor. Therefore, we carried out an ultrastructural morphometrical and immunocytochemical study on hepatocytes from mice fed on GM soybean, in order to investigate eventual modifications of nuclear components of these cells involved in multiple metabolic pathways related to food processing. Our observations demonstrate significant modifications of some nuclear features in GM-fed mice. In particular, GM fed-mice show irregularly shaped nuclei, which generally represents an index of high metabolic rate, and a higher number of nuclear pores, suggestive of intense molecular trafficking. Moreover, the roundish nucleoli of control animals change in more irregular nucleoli with numerous small fibrillar centres and abundant dense fibrillar component in GM-fed mice, modifications typical of increased metabolic rate. Accordingly, nucleoplasmic (snRNPs and SC-35) and nucleolar (fibrillarin) splicing factors are more abundant in hepatocyte nuclei of GM-fed than in control mice. In conclusion, our data suggest that GM soybean intake can influence hepatocyte nuclear features in young and adult mice; however, the mechanisms responsible for such alterations remain unknown.
Author Keywords: cell nucleus; liver; genetically modified soybean
KeyWords Plus: DIETARY NUCLEOTIDES; SMALL-INTESTINE; DNA; RATS; DEPRIVATION; PROTEIN; DISEASE; LIVER
Addresses: Malatesta M (reprint author), Univ Urbino, Ist Istol & Anal Lab, Via Zeppi S-N, Urbino, PU I-61029 Italy
Univ Urbino, Ist Istol & Anal Lab, Urbino, PU I-61029 Italy
Ist Zooprofilatt Sperimentale Umbria Marche, Perugia, I-06126 Italy
Univ Urbino, Ist Biomatemat Localita Crocicchia, Urbino, PU I-61029 Italy
Univ Urbino, Ist Chim Biol G Fornaini, Urbino, PU I-61029 Italy
Publisher: JAPAN SOC CELL BIOLOGY, SHIMOTACHIURI OGAWA-HIGASHI, KAMIKYOKU KYOTO, 602, JAPAN
Subject Category: CELL BIOLOGY
IDS Number: 617TV

ISSN: 0386-7196
被引三次,都是自引,头一次是改错,无全文下载,不知改了什么。Cell Struct Funct 2003 IF 1.664, Eur J Histochem 1.041,都是细胞生物学类百名之后的期刊。其实验还未经其它研究组重复。

Ultrastructural morphometrical and immunocytochemical analyses of hepatocyte nuclei from mice fed on genetically modified soybean (vol 27, pg 173, 2002)
Author(s): Malatesta M, Caporaloni C, Gavaudan S, Rocchi MBL, Serafini S, Tiberi C, Gazzanelli G
Source: CELL STRUCTURE AND FUNCTION 27 (5): 399-399 OCT 2002
Document Type: Correction

Fine structural analyses of pancreatic acinar cell nuclei from mice fed on genetically modified soybean
Author(s): Malatesta M, Biggiogera M, Manuali E, Rocchi MBL, Baldelli B, Gazzanelli G
Source: EUROPEAN JOURNAL OF HISTOCHEMISTRY 47 (4): 385-388 OCT-DEC 2003
Document Type: Letter
Language: English
Cited References: 14 Times Cited: 2
Abstract: We carried out ultrastructural morphometrical and immunocytochemical analyses on pancreatic acinar cell nuclei from mice fed on genetically modified (GM) soybean, in order to investigate possible structural and molecular modifications of nucleoplasmic and nucleolar constituents. We found a significant lowering of nucleoplasmic and nucleolar splicing factors as well as a perichromatin granule accumulation in GM-fed mice, suggestive of reduced post-transcriptional hnRNA processing and/or nuclear export. This is in accordance to already described zymogen synthesis and processing modifications in the same animals.
Author Keywords: cell nucleus; exocrine pancreas; genetically modified soybean; splicing factors
KeyWords Plus: CONSTITUENTS; REGIONS
Addresses: Malatesta M (reprint author), Univ Urbino Carlo Bo, Ist Istol & Anal Lab, Via Zeppi SN, Urbino, I-61029 Italy
Univ Urbino Carlo Bo, Ist Istol & Anal Lab, Urbino, I-61029 Italy
Univ Urbino Carlo Bo, Ist Biomatemat, Urbino, I-61029 Italy
Univ Pavia, Dipartimento Biol Anim, Lab Biol Cellulare, Pavia, I-27100 Italy
CNR, Ist Genet Mol, Pavia, I-27100 Italy
Ist Zooprofilattico Sperimentale Umbria & Marche, Perugia, Italy
Publisher: LUIGI PONZIO E FIGLIO, VIA D DA CATALOGNA 1/3, 27100 PAVIA, ITALY
Subject Category: CELL BIOLOGY
IDS Number: 760LJ

ISSN: 1121-760X

Ultrastructural analysis of testes from mice fed on genetically modified soybean
Author(s): Vecchio L, Cisterna B, Malatesta M, Martin TE, Biggiogera M
Source: EUROPEAN JOURNAL OF HISTOCHEMISTRY 48 (4): 449-453 OCT-DEC 2004
Document Type: Letter
Language: English
Cited References: 20 Times Cited: 0
Abstract: We have considered the possible effects of a diet containing genetically modified (GM) soybean on mouse testis. This organ, in fact, is a well known bioindicator and it has already been utilized, for instance, to monitor pollution by heavy metals. In this preliminary study, we have focussed our attention on Sertoli cells, spermatogonia and spermatocytes by means of immunoelectron microscopy. Our results point out that the immunolabelling for Sm antigen, hnRNPs, SC35 and RNA Polymerase II is decreased in 2 and 5 month-old GM-fed mice, and is restored to normal at 8 months. In GM-fed mice of all ages considered, the number of perichromatin granules is higher and the nuclear pore density lower. Moreover, we found enlargements in the smooth endoplasmic reticulum in GM-fed mice Sertoli cells. A possible role played by traces of the herbicide to which the soybean is resistant is discussed.
KeyWords Plus: VISUALIZATION; ROUNDUP; NUCLEI; RNA
Addresses: Biggiogera M (reprint author), Univ Pavia, Dipartimento Biol Anim, Lab Biol Cellulare & Neurobiol, Piazza Botta 10, Pavia, I-27100 Italy
Univ Pavia, Dipartimento Biol Anim, Lab Biol Cellulare & Neurobiol, Pavia, I-27100 Italy
CNR, Ist Genet Mol, Pavia, Italy
Univ Urbino Carlo Bo, Ist Istol, Urbino, Italy
Univ Chicago, Dept Mol Genet & Cell Biol, Chicago, IL 60637 USA
Univ Urbino Carlo Bo, Anal Lab, Urbino, Italy
E-mail Addresses: marcobig@unipv.it
Publisher: LUIGI PONZIO E FIGLIO, VIA D DA CATALOGNA 1/3, 27100 PAVIA, ITALY
Subject Category: CELL BIOLOGY
IDS Number: 883EF

ISSN: 1121-760X



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