ࡱ > % 9 R bjbj 2> s l , \ K @ X X X X X X X J J J J J J J $ L N h J ] X X X X X J X X 9K X X X J X J P 2 D RI X `LQd v nF . RI d OK 0 K F 'O 'O RI υoNlQS[N\^ew*Ovof[bJTKNN hKmP h - S A vb̃;m'`SvQTQyb̃ }vOSTb̃\O(u [vvO(ublQS͑~vؚHehvWV]z̃y cgqP H - S A cCgN)R N@b]zAmzNQ\N(W4x̃my_ Nnm-NRkxx }lmD N A ُ NǏzvv/fO g~7hT-N N+Tkxx } 6RYbёĞrat̃]zYP H - S A 7hT, hKm@b_P H - S A 7hT[2u'lgёĞrat̃M R S A vb̃;m'`TehKmekxx }kYuvP H - S A 7hTNUg0(lĂg04Yb[Ug0kxx }/f&T gOSTb̃\O(u0 [Pge 1 . ;NNhV 1 . 1 Gd^ Nwmy_:ghxvz@b, S C F - 2 4 1 . 2 Xl~ހ|x:g[leyhVxvz@bJ Y 9 8 - 3 D 1 . 3 y_:gB e c k m a n J 2 - H S 1 . 4 A K T A ˆ}vY~S|~P h a r m a c i a B i o t e c h P u r i f i e r 1 0 0 1 . 5 ~4lňnM i l l i p o r e , M i l l i Q B i o c e l 1 . 6 +}Y- SRIQIQ^P h a r m a c i a B i o t e c h U l t r o s p e c 4 0 0 0 1 . 7 K X B - 2 5 0 A uSW{Q{ NwmygՋNhVS 1 . 8 W{Qv 2 RyN(SՋBR C M S e p h a r o s e F a s t F l o w P h a r m a c i a B i o t e c h |xx0/lSI{GW:NVNA R ~ՋBR 3 OՋT 2u'lgёĞrat̃M A S A , 1uV]bu }]Nxvz@bcO l\(ukxx } 1 g 1 0 0 NUSMO / /e NSS6Ro NTybS0 4 0 1 0 2 l\(u4Yb[Ug 0 . 5 g / /e P6Ro NTybS0 4 0 9 0 0 2 1 l\(u(lĂg 0 . 5 g / /e NSS6Ro NTybSY 0 3 1 0 3 0 1 l\(uUg 0 . 5 g / /e w[^6Ro NTybS0 2 0 1 0 8 [el 1 . 6RYbёĞrat̃]zY \ёĞrat̃vOS O[YA g r D ޏc0R'Ỹ }I a vWz Nb_bN]~TbёĞrat̃]zYvTˆ}v\xbёĞrat̃]zYvD N A GrkKQeQ'YFg̃h}SOg^͑~(|lS'YFg̃[;Ñb_bؚHehbёĞrat̃]zYvWV]z̃~Ǐ͑~(|D N A ^RKm[ThNirvt[@bhvbёĞrat̃]zYNP H - S A cCgN)RhNir N0 \̃'YϑA~k4 L F A B 2 2 5 r p m 3 7 !6 h y_lm̃SO4 !6 0 0 0 g 2 0 m i n S4 !05 0 m M |xxQm+ 2 m M E D T A + 2 m M D T T 5 0 - 8 0 m L `nm̃SOX4xx4 !4 0 W 1 - 2 m i n ؚy_4xx̃SO4 !5 0 0 0 0 - 7 0 0 0 0 g 1 . 5 h 5 0 m M |xxQm2 m M E D T A 2 L gǏY\gmy_4 !3 0 0 0 0 g 1 0 m i n Nn N7hNC M yP[Nbcg4 !00 . 3 M N a C l + 5 0 m M |xxQmm1T_0RNbёĞrat̃]zY0 2 . 7hTmSm^vKm[ ˆ}v(+TϑvKm[ cgq B r a d f o r d lۏL0 2 . 1 hQf~6RY S5 /eՋ{R+RReQ2 . 5 m l leN݄nm R+R(WE p p e n d o r f {-NReQ1 m g / m l [r@n}vˆ}vB S A 1 0 l 02 0 l 04 0 l 05 0 l N~4le5 0 l ReQ NՋ{-NTeN5 0 l ~4lZPzz}v[gq am S[)n>en3 m i n Km[5 9 5 n m Y8T6e